彩票大全软件

Latency is a common strategy in a wide range of viral lineages, but its prevalence in giant viruses remains unknown. Here we describe a 617 kbp integrated giant viral element in the model green alga Chlamydomonas reinhardtii . We resolve the integrated viral genome using long-read sequencing, identify a putative polinton-like integrase, and show that viral particles accumulate primarily during the stationary growth phase. A diverse array of viral-encoded selfish genetic elements is expressed during viral activity, including several Fanzor nuclease-encoding transposable elements. In addition, we show that field isolates of Chlamydomonas sp. harbor signatures of endogenous giant viruses related to the C. reinhardtii virus that exhibit similar infection dynamics, suggesting that giant virus latency is prevalent in natural host communities. Our work describes an unusually large temperate virus of a unicellular eukaryote, substantially expanding the scope of cryptic viral infections in the virosphere.

中文翻译：

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单细胞绿藻中巨型病毒的隐匿感染


潜伏期是多种病毒谱系中的常见策略，但其在巨型病毒中的普遍性仍然未知。在这里，我们描述了模型绿藻 Chlamydomonas reinhardtii 中的 617 kbp 集成巨型病毒元件。我们使用长读长测序解析整合的病毒基因组，鉴定出一种推定的波林顿样整合酶，并表明病毒颗粒主要在静止生长期积累。在病毒活动过程中表达多种病毒编码的自私遗传元件，包括几种编码 Fanzor 核酸酶的转座元件。此外，我们表明，衣藻属的田间分离株具有与莱茵梭菌病毒相关的内源性巨型病毒的特征，这些病毒表现出相似的感染动态，表明巨型病毒潜伏期在自然宿主群落中普遍存在。我们的工作描述了一种异常大的单细胞真核生物温带病毒，大大扩大了病毒圈中隐蔽病毒感染的范围。